A reverse phase liquid chromatography (LC) method was developed and validated for simultaneous estimation of cefuroxime axetil and linezolid in pharmaceutical dosage form. The isocratic LC analysis was performed on phenomenex gemini ODS C18 column (200 mm x 4.6 mm, 5μ) using mobile phase composed of buffer pH 4.5:acetonitrile (55:45, v/v) at a flow rate of 1.0 ml/min. Quantitation was performed using UV detector at 225 nm. The retention times were found to be 4.17 min for cefuroxime axetil and 6.87 min for linezolid. The analytical method was validated according to ICH guidelines. The linearity was observed in the range of 15-45 and 12.5-37.5 μg/ml with correlation coefficient, r2=0.997 and 0.995 for cefuroxime axetil and linezolid respectively. The accuracy (%recovery) was found to be 99.88-101.81% for cefuroxime axetil and 99.79-101.89% for linezolid. The relative standard deviation values for repeatability and intermediate precision studies were less than 2%. The method was successfully applied for market sample analysis and mean percentage assay values were 98.99±0.491 and 98.636±1.379 for cefuroxime axetil and linezolid respectively. The present method is precise and accurate and can be used for the routine estimation cefuroxime axetil and linezolid in pharmaceutical dosage form.
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